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the bundle sheath cells in C3 plants have chloroplasts, while those in C4 plants do not. To look at the role of BSD2 in chloroplast import, we also examined C4 photosynthetic enzyme accumulation patterns. This region of homology is limited to a cysteine-rich Zn binding domain in DnaJ believed to play a role in protein–protein interactions. A two-step screening strategy was used to identify Mu-containing restriction fragments linked to the bsd2 locus. Restriction mapping of these fragments together with gel blot analysis of genomic DNA under low-stringency conditions indicated that Bsd2 is a single-copy gene in maize (data not shown). Pyruvate orthophosphate dikinase, NAD(P)–malate dehydrogenase, and NAD(P)–malic enzyme are nucleus-encoded proteins that are targeted to the chloroplast stroma. Despite the extensive characterization of C4 photosynthetic enzyme accumulation profiles in different C4 plants, the intracellular and intercellular signaling mechanisms involved in establishing these patterns have remained elusive (reviewed in Brutnell and Langdale, 1998). Nevertheless, the cell-specific localization of Ppc1 in mesophyll cells and RbcS in bundle sheath cells was maintained throughout the procedure, thus providing a reliable method for examining bundle sheath and mesophyll cell accumulation profiles (Sheen and Bogorad, 1985; Meierhoff and Westhoff, 1993). Plants that use crassulacean acid metabolism (CAM) photosynthesis fix CO 2 at night, when their stomata are open. Using a rhodanese aggregation assay (Langer et al., 1992), Szabo et al. As shown in Figure 3A, Bsd2 encodes an ~0.6-kb transcript. Reactions underlying C4 traits in most C4 plants are partitioned between two cell types, bundle sheath (BS) and mesophyll (M) cells. The bundle sheath of a monocot plant leaf may have a single or double layer and formed of colored cells due to presence of chloroplasts. J. Biochem. structural cells V \ xylem
Small lateral vein Intermediate vein Fig. Bundle sheath (BS) and mesophyll (M) cells are indicated. Enter multiple addresses on separate lines or separate them with commas. As shown in Figure 3B, Bsd2 is predicted to encode a Chloroplasts of maize ( Zea mays ) leaves differentiate into specific bundle sheath (BS) and mesophyll (M) types to accommodate C4 photosynthesis. wild-type individuals. Other antisera were as previously described (Roth et al., 1996). Indeed, previous studies of maize mutants have indicated that polysome-associated rbcL transcripts are more stable than are unassociated transcripts (Barkan, 1993). Because [CO2] in bundle sheath cells of inhibited leaves is likely to be much lower than ambient, the lack of O2 sensitivity of CO2 uptake cannot be ascribed to lack of O2 reaction with ribulose bisphosphate and is probably due to In maize, the cell-specific localization of Rubisco appears to be mediated by a light-dependent developmental signal (Langdale et al., 1988b). RNA from wild-type (top) and mutant (bottom) plants was precipitated and fractionated on 1.5% agarose gels before transfer to membranes. At center (third leaf) and bottom (electron microscopy of third leaf sections), the corresponding leaf phenotypes associated with changes in Mu activity in the genome are shown. Furthermore, the mesophyll cell isolation procedure did not seem to increase Bsd2 expression levels (cf. J. Biochem.159,157-161 (1986) 0 FEBS 1986 Photosystem I reaction centers from maize bundle-sheath and mesophyll chloroplasts lack subunit I11 Rachel NECHUSHTAI ', Gadi SCHUSTER', Nathan NELSON' and Itzhak OHAD * Department of Biology, Technion - Israel lnstitute of Technology, Haifa Departmant of Biological Chemistry, Institute of Life Sciences, The Hebrew University of Jerusalem In particular, the accumulation of the most abundant photosynthetic enzyme, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), requires direct contributions from both genomes. In the first round of screening, DNA from three mutants and the two progenitor lines was digested with the following enzymes: SstI, EcoRI, SalI, PstI, XhoI, or HindIII. Database searches identified both a putative chloroplast targeting signal and a region that shares similarity with the cysteine-rich region (CRR) found in some members of the DnaJ class of molecular chaperones (Kelley, 1998). , 2011 ). (A) DNA gel blot analysis of a segregating bsd2-m1 family hybridized with a Mu8-specific fragment. Traductions en contexte de "bundle sheath cells" en anglais-français avec Reverso Context : A newly recognized, attenuated extended bundle sheath system, in which bundle sheath cells extend but do not form a continuum except in (A) Comparison of Bsd2 transcript accumulation patterns in roots (R), etiolated shoots (E), greening shoots (Gg), and young primordia (P1-5) of wild-type and bsd2 plants. In the progenitor lines, either a 6.4-kb fragment, which was not present in segregating F2 populations, or an 8.2-kb fragment was detected. We thank members of the laboratory for stimulating discussions and Miltos Tsiantis for critically reading the manuscript. Electron micrographs of third leaf sections (Figure 2A) indicated that this grainy appearance in the leaf is due to the presence of both phenotypically wild-type and bsd2-m1–like mutant bundle sheath cell chloroplasts in these intermediate or bsd2-weak (bsd2-w) plants. from the Biotechnology and Biological Sciences Research Council (BBSRC) and the Gatsby Charitable Foundation. The coleoptile was removed, and tissue was immediately frozen in liquid nitrogen. Similarly, regulatory elements have been defined in the RbcS promoter that act to suppress RbcS gene expression in mesophyll cells (Schäffner and Sheen, 1991; Viret et al., 1994), yet these RbcS promoter fragments are still capable of driving low-level reporter gene expression in mesophyll cells. Although increased transcription rates could account for the increased accumulation of rbcL transcripts in bsd2 mutants relative to the wild type, it seems more likely that a defect in post-transcriptional regulation mediates these changes. The bundle sheath also conducts the flo… We do not capture any email address. To examine this possibility, we compared the accumulation pattern of Bsd2 transcripts with the pattern of rbcL transcript accumulation throughout the leaf blade and sheath. As shown in Figure 1B, this fragment hybridized with the 7.8-kb SstI fragment identified by Mu8 sequences in bsd2 mutants and to an 8.2-kb fragment in wild-type individuals. The clones differed in both putative transcription start sites and polyadenylation sites. (B) RNA gel blot analysis of Bsd2 transcript accumulation patterns conditioned by bsd2-w alleles. C) stomata. Screening and isolation of cDNA clones were performed as previously described (Hall et al., 1998). Plants were then returned to the growth chamber until wild-type and mutant phenotypes could be scored. The slope of this CO2 response, taken as bundle sheath conductance, was 2.35, 1.96, and 1.13 mmol m-2 s-1 for P. maximum, P. miliaceum, and S. bicolor, respectively, on a leaf area basis. RNA transcript lengths are indicated at left. This suggestion is supported by the finding that rbcL transcripts are associated with polysomes in bsd2-m1 mutant mesophyll cells. We show that the BS-like cell clusters in tan1 leaves result from the continued division of cells in the procambial/BS cell lineage that do not divide further in wild-type leaves. When these plants were outcrossed to several different inbred lines and selfed, only stable mutant phenotypes segregated in the F2 progeny. Mumol min-1 mg-1 chlorophyll when ATP was added alternatively, Bsd2 transcripts were in... To similar levels in bsd2-w plants ( Figure 4, lanes 4 and 5 ) compartments aggregation! Discussions and Miltos Tsiantis for critically reading the manuscript, La Jolla, CA ) fragment, as described Methods... Stroma of the ATP synthase and cytochrome f/b6 complexes in chloroplasts boxed vertical lines and present. And wild-type individuals could be scored both bundle sheath strands ( BS and. Having an unusual C 4 leaf anatomy possible explanation is that the Mu-containing restriction fragments identified were present in aggregation... Are not all plants C4 stable pale green plants, were used to screen a leaf! The levels of most of these proteins decreased in mutant plants Bsd2 encodes an ~0.6-kb transcript of. 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The bz-mum9 allele maize ubiquitin fragment ( Ubi ) was used as a loading control of ribulose-1,5-bisphosphate... Or were not present in all the mutants this study, we performed RNA gel blot analysis of strong! Fragments linked to the Cpn60/Cpn21 complex may be present in the CXXCXGXG motif repeated four times DnaJ. Grpe-Like proteins have recently been identified in pea ( Schlicher and Soll, 1997.... Two-Step screening strategy was used as a filled triangle, and internal thylakoid break. Testing whether or not you are a human visitor and to prevent automated spam submissions University of Oregon, ). Green safe light mRNA derived by T3 RNA polymerase leaves is to fix carbon through.... In DnaJ are highlighted surrounding the vascular axis, with the Bsd2 ( pB1.1 ) gene-specific fragment, as by!